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. 2009 Sep 25;136(20):3495–3504. doi: 10.1242/dev.036087

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Fig. 5. — Progressive recovery of myotomal basement membrane in Gli3^-/-;Shh^-/- embryos. (A-I) β1 laminin subunit (green) and Myf5 (red) protein distribution examined by immunohistochemistry and confocal imaging in somites of E10.0 Shh^-/- (A-C), Shh^-/-;Gli3^-/- (D-F), and wild-type (G-I) embryos. (A-C) Shh^-/- embryos. (D-F) Shh^-/-;Gli3^-/- somites. Note the recovery of epaxial Myf5 activation at all axial levels (white asterisk). (G-I) Wild-type embryos. Yellow arrowheads indicate the dermomyotomal basement membrane. Red arrows show abnormally located myotomal cells. White arrows indicate sites of partially assembled basement membrane. Magnification: ×400.