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. 2009 Sep 25;136(20):3495–3504. doi: 10.1242/dev.036087

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Fig. 6. — Exogenous laminin-111 restores myotomal basement membrane formation. (A-D) Laminin (green) and Myf5 (red) distribution in E10.5 Shh^-/-;Gli3^-/- embryos cultured for 12 hours in control medium (A,B) or in the presence of laminin-111 (30 μg/ml) (C,D). (A) No myotomal basement membrane forms in Shh^-/-;Gli3^-/- hindlimb somites, whereas (B) a higher degree of organisation is observed in Shh^-/-;Gli3^-/- interlimb somites in control medium. By contrast, Shh^-/-;Gli3^-/- hindlimb (C) and interlimb (D) somites form a myotomal basement membrane when cultured in the presence of laminin-111 (red arrows). Yellow arrowheads indicate the dermomyotomal basement membrane. Magnification: ×200.