Table 1.

Inhibitory strength of brachialactone on AMO or HAO enzymatic pathways of N. europaea

Compound	Concentration in the in vitro assay, μM	Inhibition, %
		AMO pathway	HAO pathway
BNI-root exudate (crude methanol extract)	—	63.4 ± 0.8	63.8 ± 0.8
Brachialactone	5.0	59.7 ± 0.9	37.7 ± 0.9
Nitrapyrin	3.0	82.3 ± 1.5	8.1 ± 1.2

Root exudate was collected from intact BH (CIAT 679) plants (root fresh weight of ≈20 g) using aerated solutions of 1 mM NH₄Cl, evaporated to dryness, extracted with methanol, and evaporated to dryness and dissolved in 200 μL of dimethyl sulfoxide; 1 μL of the crude extract was used for the determination of BNI activity. Values are means ± SE from four replications.