Fig. 5.

TAP is not required for MHC class I presentation of HEL in early endosomal liposomes. (A and B) Peritoneal Mφs from WT and TAP^−/− B6 mice were incubated with antigen and CD8 T-cell hybridomas. Peritoneal Mφs (C) and splenic DCs (D) from TAP^−/− mice were incubated with Flu-NP or LLO peptide at 26 °C. After 12–16 h, HEL in DOPE/CHEMS liposomes and CD8 T-cell hybridomas were added overnight at 37 °C and IL-2 was measured in culture supernatants. Hybridoma responses to HEL 23–31 peptide were unaffected by addition of Flu-NP or LLO peptide.