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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4917–4930. doi: 10.4049/jimmunol.0803050

FIGURE 3.

FIGURE 3

FNBP1L interacts with ATG3 via the FNBP1L HR1 domain and locates to internalized bacteria. A, Schematic representation of FNBP1L constructs used in this study. FCH, FER/CIP4 homology (FCH) domain; CC, coiled-coil domain; HR1, protein kinase C-related kinase homology region 1; SH3, Src homology 3 domain. B, Full-length FNBP1L or FNBP1L mutants and ATG3 were expressed in HEK293T cells either together or individually as indicated. Twenty-four hours after transfection the expressed proteins were immunoprecipitated (IP) and probed with the indicated Abs. FNBP1L/ATG3 binding was observed to be HR1 domain dependent. HC, Heavy chain; KD, kDa.

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