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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Oncogene. 2009 Mar 23;28(16):1821–1832. doi: 10.1038/onc.2009.31

Figure 4. The Src phosphorylation site on EGFR, Y845, is important for constitutive activation of mutant EGF receptors.

Figure 4

(A) Cells expressing the indicated EGFRs were serum-deprived for 3 hr and then either left unstimulated (−) or stimulated (+) with 10 ng/ml EGF for 10 min., or (B) Cells were placed in media with 10 % FBS (Normal Growth Condition) or 0.1 % FBS for 3 hr (3 hr serum-deprivation). 50 μg aliquots of whole cell lysates were used for immunoblotting with antibodies against the indicated proteins.