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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Oncogene. 2009 Mar 23;28(16):1821–1832. doi: 10.1038/onc.2009.31

Figure 6. Src and its phosphorylation on EGFR Y845 is critical for mutant EGFR-mediated cell migration.

Figure 6

Transwell migration was performed on cells expressing the indicated EGFRs (A) or indicated Src (C) with or without 10ng/ml EGF on the bottom chamber. After 16 hr, cells were fixed, their nuclei stained with propidium iodide and pictures were taken with a fluorescence microscope. Each condition was prepared in triplicate with four high power fields per replicate. (B) The number of cells migrated from (A) or (D) number of cells migrated from (C) were quantified using the ImageJ software. Data points represent the average number of migrated cells in four high power fields (HPF). Data were expressed as number of migrated cells per HPF (mean ± S.D.). Analysis of Variance (ANOVA) performed on the number of migrated cells yielded a statistically significant difference between cell types.