Table 1.
List of the 78 peptides identified in the in vivo formed Human Acquired Enamel Pellicle.
| Accession number1 |
Protein name | Peptide identified (sequence domain within parent protein) | MW (Da) |
pI | Protein region | Times identified |
|---|---|---|---|---|---|---|
| P02810 | Salivary acidic proline-rich phosphoprotein 1/2 | F.IDEERQGPPLG.G (42–52)a,b | 1211.3 | 3.8 | N-terminal/Middle | 2 |
| F.IDEERQGPPLGGQQ.S ▪ (42–55)a,b | 1524.6 | 3.8 | N-terminal/Middle | 3 | ||
| F.IDEERQGPPLGGQQSQPS.A (42–56)b | 1924.0 | 3.8 | N-terminal/Middle | 2 | ||
| G.GQQQQGPPPPQGKPQ.G (78–92)a,b | 1572.7 | 10.1 | Middle | 3 | ||
| G.PQQGPPQQGGQQQQGPPPPQG.K (53–73)a,b | 2137.3 | 6.0 | Middle | 3 | ||
| K.PQGPPPQGGRPQGPPQ.G (130–145)a,b | 1595.7 | 11.0 | C-terminal | 3 | ||
| N.QDDGPQQGPPQQGGQQQQGPPPPQGKP.Q(49–75)a,b | 2777.9 | 3.9 | N-terminal/Middle | 4 | ||
| P.PPGKPQGPPPQGGR.P(126–139)a,b | 1370.5 | 11.5 | C-terminal | 4 | ||
| Q.DDGPQQGPPQQGGQQQQGPPPPQGKPQ.G(50–76)a,b | 2777.9 | 3.9 | N-terminal/Middle | 3 | ||
| Q.GGRPQGPPQGQSPQ.-(137–150)a,b | 1391.5 | 11.0 | C-terminal | 4 | ||
| Q.GPPPPPPGKPQGPPPQ.G(121–135)a,b | 1545.8 | 10.1 | C-terminal | 3 | ||
| Q.GPPPPPPGKPQGPPPQGGRPQGPPQGQSPQ.-(121–150)b | 2918.2 | 11.5 | C-terminal | 2 | ||
| Q.GPPQQGGHQQGPPPPPPGKPQ.G (111–131)▪•a,b | 2084.3 | 10.5 | C-terminal | 4 | ||
| P02812 | Basic salivary proline-rich protein 2 | K. PQGPPPQGGNKPQGPPPPGK.P(23–43)a,b | 2810.1 | 10.6 | N-terminal | 3 |
| G.GNQPQGPPPPPGKPQ.G(278–292)a,b | 1496.7 | 10.1 | C-terminal | 3 | ||
| K.PQGPPPQGGNKPQGPPPPGK.P(167–186)a,b | 1933.2 | 10.6 | C-terminal | 3 | ||
| K.PQGPPPQGGNKPQGPPPPGKPQGPPPQGDK.S(167–196)a | 2935.2 | 10.3 | N-terminal | 2 | ||
| K.PQGPPPQGGNQPQGPPPPPGKPQ.G(84–106)a,b | 2255.5 | 10.1 | N-terminal | 3 | ||
| P.GKPQGPPPQGGNQPQGPPPPPGKPQG.P*(227–251)b | 2497.8 | 10.6 | C-terminal | 2 | ||
| Q.GPPPQGGNKPQGPPPPGKPQ.G(107–126)a,b | 1922.2 | 10.6 | N-terminal | 3 | ||
| Q.GPPPQGGNQPQGPPPPPGKPQ.G(147–168)a,b | 2030.2 | 10.1 | C-terminal | 4 | ||
| Q.GPPPQGGNQPQGPPPPPGKPQGPPPQGGNKPQ.G(147–179)a,b | 3088.4 | 10.6 | C-terminal | 3 | ||
| P02808 | Statherin | F.GYGYGPYQPVPE.Q ▪ (15–26)a,b | 1326.4 | 3.3 | Middle | 3 |
| -.DSSEEKFLR.R (1–9)a,b | 1110.2 | 4.4 | N-terminal | 2 | ||
| R.IGRFGYGYGPYQPVPEQP.L (11–28)a,b | 2025.2 | 6.8 | N-terminal/Middle | 3 | ||
| R.RIGRFGYGYGPYQPVPEQPLYPQPYQPQYQQYT.F(11–42)a,b | 3981.4 | 9.1 | N-terminal/Middle/C-terminal | 3 | ||
| Y.GPYQPVPEQPLYPQPYQPQ.Y▪ (19–37)a,b | 2226.4 | 3.3 | Middle/C-terminal | 4 | ||
| P04745 | alpha-amylase A1(salivary) | R.SGNEDEFR.N (88–95)a,b | 953.9 | 3.8 | N-terminal | 4 |
| R.ALVFVDNHDNQR.G (307–318)b | 1428.5 | 5.1 | Middle | 2 | ||
| F.IYQEVIDLGGEPIK.S (245–258)a,b | 1574.8 | 3.8 | Middle | 3 | ||
| K.AHFSISNSAEDPFIAI.H(490–505)a,b | 1719.9 | 4.1 | C-terminal | 4 | ||
| K.TGSGDIENYNDATQVR.D(158–173)a,b | 1740.8 | 3.7 | N-terminal | 3 | ||
| Q9hcy8 | S100 calcium binding protein a14 | K.NFHQYSVEGG.K(28–37)a,b | 1137.1 | 5.1 | N-terminal | 3 |
| S.FWELIGEAAK.S (84–93)a,b | 1163.3 | 4.3 | C-terminal | 3 | ||
| F.RSFWELIGEAAKSVKLE.R(82–98)a,b | 1963.2 | 7.2 | C-terminal | 2 | ||
| A.QEFSDVERAIETLI.K(13–26)a,b | 1649.8 | 3.7 | N-terminal | 4 | ||
| K.IANLGSCNDSKL.E(68–78)a,b | 1234.3 | 6.1 | Middle/C-terminal | 3 | ||
| P04083 | Annexin A1 | F.IENEEQEYVQTVK.S (14–26)a,b | 1608.7 | 3.8 | N-terminal | 4 |
| K.TPAQFDADELR.A(114–124)a,b | 1262.3 | 3.7 | Middle | 3 | ||
| W.FIENEEQEYVQTVK.S (13–26)a,b | 1755.9 | 3.8 | N-terminal | 3 | ||
| Q9UBG3 | Corunlin | R.TEGNCTALTRGE.L (21–32)a,b | 1251.3 | 4.3 | N-terminal | 4 |
| E.GNCTALTRGELKR.L(23–35)a,b | 1418.6 | 10.1 | N-terminal | 3 | ||
| R.EQGQTQTQPGS.G(373–383)a,b | 1160.1 | 12.4 | C-terminal | 4 | ||
| P01040 | Cystatin a | E.KTNETYGKLE.A (30–39)a,b | 1182.3 | 7.0 | Middle | 4 |
| E.TYGKLEAVQY.K (34–43)a,b | 1171.3 | 6.8 | N-terminal/Middle | 2 | ||
| K.SLPGQNEDLVLTG.Y (72–84)a,b | 1342.4 | 3.0 | C-terminal | 3 | ||
| P04280 | Basic salivary proline-rich protein 1 | A.GNPQGPSPQGGNKPQ.G(18–32)▪a,b | 1462.5 | 10.1 | N-terminal | 3 |
| Q.GPPQQGGNRPQGPPPPGKPQ.G(226–245)▪b | 1991.2 | 11.5 | Middle/C-terminal | 3 | ||
| A.GNPQGPSPQGGNKPQGPPPPPGKPQ.G(18–48)▪b | 2415.6 | 10.6 | N-terminal | 4 | ||
| P08779 | Cytokeratin-16 | R.EVFTSSSSSSSRQ.T (442–454)a,b | 1388.4 | 7.0 | C-terminal | 3 |
| P15515 | Histatin-1 | F.YGDYGSNYLYDN.-▪(27–38)a,b | 1443.4 | 2.9 | Middle/C-terminal | 3 |
| Y.GDYGSNYLYDN.-▪• (28–38)a,b | 1280.2 | 2.9 | C-terminal | 4 | ||
| Q96QV6 | Histone H2A type1-A | I.AQGGVLPNIQAV.L (104–115)b | 1166.3 | 6.0 | C-terminal | 2 |
| V.TIAQGGVLPNIQAV.L (102–115)a,b | 1380.6 | 6.0 | C-terminal | 4 | ||
| Q9HC84 | Mucin-5B | L.SSPSPAPGCDNAIP.L (4940–4953)a,b | 1312.4 | 3.1 | C-terminal | 2 |
| R.AQAQPGVP.L (2362–2369)a | 766.9 | 6.0 | C-terminal | 2 | ||
| P62736 | Actin, aortic smooth muscle | Y.VGDEAQSKRGILTL.K (56–69)a,b | 1487.7 | 7.0 | N-terminal | 3 |
| T.AASSSSLEKSYELPDGQVI.T (232–250)a | 1982.1 | 3.8 | N-terminal | 2 | ||
| P06733 | Alpha-enolase | A.NGWGVMVSH.R (363–371)a,b | 986.1 | 7.8 | C-terminal | 4 |
| R.SERLAKYNQLLRIEEE.L(401–416)a,b | 1991.2 | 4.7 | C-terminal | 3 | ||
| P06702 | Protein S100-A9 | Y.SVKLGHPDTLNQGEFKEL.V (23–40)a,b | 2013.2 | 5.3 | N-terminal/Middle | 3 |
| V.KLGHPDTLNQGEF.K (25–37)a,b | 1456.6 | 5.2 | N-terminal/Middle | 3 | ||
| Q01546 | Cytokeratin-2P | R.GVFGGVSGSGSGGYK.G (524–538)a,b | 1316.4 | 9.7 | C-terminal | 4 |
| K.SGGGGSTSIRFSQTTSSSQHSSTK.- (615–638)a,b | 2373.4 | 11.5 | C-terminal | 4 | ||
| P02768 | Serum albumin | K.PLVEEPQNLIK.Q (403–413)a,b | 1279.5 | 4.3 | Middle/C-terminal | 3 |
| K.AVMDDFAAFVEK.C (570–581)b | 1342.5 | 3.7 | C-terminal | 2 | ||
| P04406 | Glyceraldehyde-3-phosphate dehydrogenase | A.SEGPLKGILGY.T (266–276)a,b | 1133.3 | 6.9 | C-terminal | 4 |
| V.SSDFNSDTHSSTF.D(283–295)a,b | 1431.3 | 3.9 | C-terminal | 3 | ||
| P02647 | Apolipoprotein A-I | L.REQLGPVTQEF.W (85–95)a,b | 1304.4 | 4.3 | N-terminal/Middle | 3 |
| P02788 | Lactotransferrin | R.ESTVFEDLSDEAER.D (230–243)a,b | 1626.6 | 3.4 | N-terminal | 3 |
| P20671 | Histone H2A type 1-D | I.RNDEELNKLLGKVTIA.Q(89–104)b | 1814.1 | 7.1 | Middle/C-terminal | 2 |
| P01833 | Polymeric- immunoglobulin receptor | R.ASVDSGSSEEQGGSSRAL.V(623–640)a,b | 1724.7 | 3.8 | C-terminal | 4 |
| P31151 | S100 calcium- binding protein A7 | Y.HKQSHGAAPCSGGS.Q (87–100)a,b | 1323.4 | 9.0 | C-terminal | 3 |
| P47929 | galectin-7 | V.GGDVQLDSVRIF.- (125–136)b | 1305.5 | 3.9 | C-terminal | 2 |
| P30044 | peroxiredoxin-5 | M.APIKVGDAIPAVEV.F (54–67)a | 1378.6 | 4.1 | N-terminal/Middle | 2 |
| P04080 | cystatin B | Y. QTNKAKHDELTYF.- (86–98)a,b | 1594.7 | 7.7 | C-terminal | 3 |
| P01036 | Cystatin-S | Y.EVPWEDRMSLVN.S (124–135)a,b | 1474.7 | 3.8 | C-terminal | 3 |
| P05164 | Myeloperoxidase | R.DFVNCSTLPALNLASW.R (726–741)a,b | 1751.1 | 3.1 | C-terminal | 4 |
1
Accession numbers in bold represent AEP proteins identified earlier by our group using in-gel trypsinization followed by LC-ESI-MS/MS (Siqueira et al., 2007).
•
Peptides identified by Vitorino et al., 2008 in in situ formed AEP.
▪
Peptides identified by Helmerhorst et al., 2008 in human whole saliva.
a
Peptides in samples collected with dry collection strips.
b
Peptides which in samples collected with 3% citric acid-soaked collection strips.