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. Author manuscript; available in PMC: 2009 Sep 25.
Published in final edited form as: Biochemistry. 2009 Feb 24;48(7):1604–1612. doi: 10.1021/bi801888j

Figure 4. Restricted H-D exchange of PE peptide domains in the presence of TFE (trifluoroethanol).

Figure 4

H-D exchange was conducted in the presence of TFE (15%, trifluoroethanol), an organic solvent, to represent a more hydrophobic environment compared to without TFE. The H-D exchange period in D2O was 10 secs (same time as in fig. 3, without TFE). Different ranges of the percent deuteration is illustrated in a color-coded manner (see key in figure). Comparisons of percent deuteration of PE peptide domains in the absence and presence of TFE revealed changes in H-D exchange, represented by reduced deuteration of the majority of PE domains in the presence of TFE.