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. Author manuscript; available in PMC: 2009 Sep 25.
Published in final edited form as: Biochemistry. 2009 Feb 24;48(7):1604–1612. doi: 10.1021/bi801888j

Figure 5. Relative accessibilities of dibasic cleavage sites to the aqueous solvent environment are altered in the presence of TFE, as revealed by H-D exchange.

Figure 5

The average level of deuteration of each dibasic cleavage site in the presence of TFE (15%) was subtracted from that in the absence of TFE. These differences are illustrated in the bar graph plots. Reduced deuteration in the presence of TFE was observed for most cleavage sites, especially for sites #2 and #8. Modest increases in percent deuteration were observed for dibasic sites #1 and #9. These data illustrate differential effects of TFE to modify relative accessibilities of PE dibasic cleavage sties to the aqueous environment.