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SDS-PAGE analysis of recombinant RsHTMT. Proteins were separated by SDS-PAGE and stained using Coomassie brilliant blue. M, Molecular marker; Lane 1, crude cell free extract of E. coli BL21(DE3); Lane 2, crude cell free extract of E. coli transformant possessing pET-Rshtmt; Lane 3, recombinant RsHTMT purified by Ni-Sepharose resin column chromatography.
