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. Author manuscript; available in PMC: 2009 Sep 25.
Published in final edited form as: Genes Brain Behav. 2009 May 20;8(5):500–511. doi: 10.1111/j.1601-183X.2009.00499.x

Figure 2. Mauthner axons fail to cross the midline in twitch twice/robo3.

Figure 2

Immunostaining with the 3A10 antibody in 48 hpf embryos highlights the Mauthner cell and axons. In wildtype sibling embryos (a) both Mauthner axons cross the midline and project caudally. In twitch twice/robo3 mutants (b,c) one or both Mauthner axons fail to cross the midline and project to ipsilateral spinal cord. At 6 dpf, immunostaining with the 3A10 antibody reveals a characteristic ladder of commissures (white triangles) and the axon cap of the Mauthner cell (black triangles) in wildtype larvae (d). Commissures are disorganized in twitch twice/robo3 mutants although the Mauthner axon cap is intact (e).