Fig. 3.

Bortezomib enhances IFN-γ-induced gene transcription in melanoma cells. Real-time PCR was used to analyze the transcript levels of IRF1, a known IFN-γ-responsive gene in A375 cells following an 18-h pre-treatment with bortezomib, followed by a 4-h stimulation with IFN-γ (10 ng/ml). Data are presented as the mean fold increase in IRF1 vs. PBS-treated cells. Data are normalized to β-actin (housekeeping gene). Error bars are derived from triplicate wells. Asterisk denotes significant difference as compared to IFN-γ-treated cells