Fig. 1.

Ethidium bromide stained agarose gel image following random mutation capture analysis. Lanes 1 and 8: full length band following two rounds of digestion, this band would be excised and gel extracted for sequencing as it is presumed mutant. Lanes 3 and 9: PCR product which has cut during the second round of digestion with Taq1α. This represents a molecule which has not been digested during the first restriction digest and so has amplified during PCR. This shows the second round of digestion to be necessary so as to identify only true mutants, and avoid unnecessary sequencing. Lane 12: Control DNA from this subject which did not undergo digestion prior to PCR, but does digest after PCR, which shows that this subject does not have a polymorphic variant in the restriction site.