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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Aging Cell. 2009 Jul 9;8(5):604–606. doi: 10.1111/j.1474-9726.2009.00503.x

Fig. 1.

Fig. 1

Calorie restriction alters mitochondrial acetylation. (A) Liver mitochondrial acetylation in CR. Mitochondrial extracts were generated from either CR or AL mice, fractionated by SDS-PAGE, and probed with the indicated antibodies. Ac-K, anti-acetyl-lysine. p=polyclonal, m=monoclonal. (B) Tissue-specific changes in mitochondrial acetylation. Mitochondria were prepared from indicated tissues of CR and AL mice and probed as in panel A. (C) Identification of proteins hyperacetylated in CR. Total acetylated proteins were immunopurified from AL and CR mitochondrial extracts and probed for the indicated proteins. Each panel represents mitochondrial extracts derived from a single AL/CR pair and is representative of at least three independent experiments.