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. 2009 Aug 24;106(38):16293–16297. doi: 10.1073/pnas.0908009106

Fig. 1.

Fig. 1.

Normalcy of ntTS cell lines. (A) ntTS and TS (control) cell lines were cultured in the presence (stem, day 4) and absence (diff, day 2 and day 4) of FGF4 and MMC-EMFI conditioned medium. (Scale bar, 0.2 mm.) (B) Northern blot analysis of ntTS and TS cells in stem (s) and differentiation (diff) conditions. (C) Established cell lines. Asterisks (*) indicate the cell lines with mild aneuploidy. (D) Correlation coefficient of Affymetrix expression microarray intensity values among the ntTS and TS cell lines. (E) MS-qPCR using the primers for TS-specific methylation loci, unmethylated loci, and Dnmt target loci. (F) Correlation coefficient of D-REAM-normalized logMED values among the ntTS and TS cell lines. (G) Density plot of absolute variance in each single comparison. Note that the differences between ntTS and TS cell lines (blue lines) are similar to the difference between the 2 TS controls (black line).