Fig. 1.

NDPK A and B depletion in zebrafish embryos. Zebrafish embryos were injected with MO-Ctrl (4 ng), MO-NDPK A (8 ng) and MO-NDPK B (4 ng). (A) Immunoblot analysis of lysates prepared 72 hpf using specific antibodies against total fish NDPK (NDPK*), NDPK B, Gβ₁ and Gγ₂. β-actin served as loading and specificity control. (B) Relative NDPK activity (normalized to MO-Ctrl) was quantified as formation of ³H-GTP from ³H-GDP and ATP in lysates of embryos injected as indicated. Data are means ± SEM, n = 3, *, P < 0.05 vs. MO-Ctrl. (C) Co-immunoprecipitation of NDPK B and Gβ in zebrabrafish lysates. Endogenous Gβ or NDPK B was immunoprecipitated (IP) from zebrafish lysates. Association of NDPK B or Gβ was detected by immunoblotting (IB). Representative immunoblots are shown.