Fig. 2.

Cardiac dysfunction in NDPK B-depleted zebrafish embryos. Cardiac phenotype of wild-type (WT) (A) and NDPK B knockdown (B) embryos at 72 hpf. Fractional shortening (FS) of the atrial and ventricular chamber of WT (C) and NDPK B morphants (D) at 48 and 72 hpf. Data are means ± SEM, n = 11–21. Histology sections of a WT (E) and NDPK B knockdown (F) heart region stained with hematoxylin/eosin. A, atrium, V, ventricle, en, endocardial, my, myocardial layer. (G–J) Whole-mount immunofluorescence following staining with specific antibodies against atrial myosin heavy chain [S46, (G) and (I)] and entire heart tube myosin heavy chain [MF20, (H and J)] showing unaltered expression of structure proteins.