Fig. 7.
Rescue of the phenotype of NDPK A/B double-knockout MEFs by adenoviral expression of human NDPK B. NDPK A/B (−/−) MEFs were infected with the adenoviruses Ad-EGFP, Ad-NDPK A and Ad-NDPK B at the multiplicity of infection (MOI) of 500. (A) Representative immunoblots of the membrane fraction of (−/−) MEFs infected as indicated confirmed the re-expression of NDPK A and B and showed elevated levels of caveolin-1, Gβ1 and Gαs compared with EGFP control infected cells. (B) cAMP production in (−/−) MEFs following infection with the adenoviruses at the indicated MOIs. Data are means ± SEM, n = 3, *, P < 0.05, **, P < 0.001 vs. Ad-EGFP infected (−/−) MEFs.