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. 2009 Sep 14;106(38):16084–16089. doi: 10.1073/pnas.0904535106

Fig. 2.

Fig. 2.

The 9-GHz EPR spectra of the D179W+R258E+R272D variant of CiP in the resting state (gray trace) and on reaction with hydrogen peroxide (green and red traces). The yield of the very broad and axial spectrum of the [Fe(IV)=O Por•+] intermediate was 5 times higher at pH 4.0. The yield of the narrow [Fe(IV)=O Trp•] species contributing at g ≈ 2 was similar at both pHs, indicating a higher conversion to the EPR-silent [Fe(IV)=O] species. Experimental conditions: 4 K; modulation amplitude, 2 G; modulation frequency, 100 kHz.