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The reaction of the D179W+R258E+R272D variant of CiP with VA. The enzyme was premixed with 2-fold excess VA before the reaction with hydrogen peroxide (10-s mixing time at 22 °C). The formation of the [Fe(IV)=O Por•+] intermediate (broad and axial EPR signal recorded at 4 K, black trace) was not affected by the presence of VA, but the Trp• signal was clearly absent (black trace; Inset).
