Table 1.
24 Cartilage relevant genes. Primers were designed Primer3 software (www.genome.wi.mit.edu/cgi-bin/primer/primer3www.cgi). Standard dilutions were used to calculate relative mRNA copy number.
| Matrix Molecules | Proteases | Protease inhibitors | Cytokines | Growth Factors | Transcription Factors | Stress Activated Genes | Housekeeping Gene |
|---|---|---|---|---|---|---|---|
| Type II Collagen | MMP1 | Timp-1 | TNF-a | IGF-1 | c-Jun | HSP90 | 18s |
| Aggrecan | MMP3 | Timp-2 | IL-1 | IGF-2 | c-Fos | Txnip | |
| Link Protein | MMP13 | Timp-3 | IL-4 | TGF-B | Sox-9 | ||
| Fibronectin | ADAMTS-5 | IL-6 |