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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Hepatology. 2009 Jul;50(1):216–229. doi: 10.1002/hep.22915

Figure 6. Protection against growth factor withdrawal-induced apoptosis in S6K1-deficient hepatocytes.

Figure 6

S6K1+/+ and S6K1−/− immortalized hepatocytes were incubated in serum-free medium for 8 and 16 h. A. Representative phase-contrast microscopy images of S6K1+/+ and S6K1−/− hepatocytes after 16 h of serum withdrawal. B. Representative images of nuclear morphology of untreated and treated S6K1+/+ and S6K1−/− hepatocytes after staining of DNA with DAPI and analysis by fluorescence microscopy. C. The percentage of cells with DNA lower than 2C (apoptotic cells) was determined by flow cytometry Results are expressed as percentage of apoptotic cells are means ± SE. Statistical significance was determined by Student's t test comparing the S6K1−/− condition with the respective values for wild-type controls. *p<0.05 was considered significant. D. Extranuclear DNA, obtained from untreated and treated S6K1+/+ and S6K1−/− hepatocytes, was electrophoresed and visualized by UV fluorescence. A representative experiment is shown. E. Representative phase-contrast micrographs of S6K1+/+ and S6K1−/− primary hepatocytes after 16 h of serum withdrawal.