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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Mol Microbiol. 2009 Aug 24;73(6):1171–1185. doi: 10.1111/j.1365-2958.2009.06846.x

Figure 4.

Figure 4

Episomal var promoters downregulate endogenous stevor and Pfmc-2TM expression. (a) Change in stevor RNA levels following increase in copy number of pVBH. Expression levels are shown of endogenous stevor genes in pVBH/B3 parasites under low (black bars) and high (grey bars) blasticin doses. Untransfected B3 parasites (carrying no episome) are represented by white bars. All RNA samples were taken at 24 hours post invasion. (b) Representation of data depicted in (a). Total stevor transcript levels of untransfected B3 parasites (white bar) and pVBH transfected B3 parasites under low (black bar) and high blasticidin doses (grey bar). The approximate copy number of the transfected episome for each drug concentration is shown in parentheses. (c) Change in Pfmc-2TM RNA levels following increase in copy number of pVBH. Expression levels are shown of endogenous Pfmc-2TM genes in pVBH/B3 parasites under low (black bars) and high (grey bars) blasticin doses. All RNA samples were taken at 28 hours post invasion. (d) Representation of data depicted in (c). Total Pfmc-2tm transcript levels of untransfected B3 parasites (white bar) and pVBH transfected B3 parasites under low (black bar) and high blasticidin doses (grey bar). The approximate copy number of the transfected episome for each drug concentration is shown in parentheses. Relative copy numbers are measured with respect to the control housekeeping gene P61-fructose bisphosphate aldolase (PF14_0425) using the formula 2−ΔΔCT with NF54 gDNA as the calibrator.