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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Mol Microbiol. 2009 Aug 24;73(6):1171–1185. doi: 10.1111/j.1365-2958.2009.06846.x

Figure 7.

Figure 7

Active var and rifin promoters colocalize at a transcriptionally active subnuclear spot. For DNA-FISH, the nuclei are stained with DAPI (blue), and the pRBH episomes are detected with a bsd probe (green). Endogenous var genes (PFD1005c-on, or PFL0030c (CSA)-off) were detected with gene-specific probes (red). Ring stage parasites transfected with pRBH were probed with the bsd probe plus either the PFD1005c-on, or PFL0030c-off probe (a) Examples of cells scored as colocalizing. (b) Examples of cells scored as non-colocalizing. (c) Quantification of colocalization of the active episome (green) or the control gene sbp1 with either the active var gene (PFD) or inactive var gene (CSA) (red) in each parasite line. Percentages and cell numbers are an average of three separate, blinded counts. (d) Graphical representation of data in (c). Black bars represent colocalization with active var gene (PFD). Grey bars represent colocalization with inactive var gene (CSA).