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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Chem Biol. 2009 Sep 25;16(9):971–979. doi: 10.1016/j.chembiol.2009.07.012

Figure 4. 13C and 1H NMR spectra of [1′-13C]inosine with human PNP in the absence of phosphate.

Figure 4

Panel I, 13C NMR spectra of [1′-13C]inosine with native human PNP after (A) 30 min and (B) 24 h.

Panel II, 1H NMR spectra of [1′-13C]inosine with native human PNP after (A) 30 min, (B) 3 h, (C) 6 h, (D) 12 h, and (E) 24 h.

Panel III, 1H NMR spectra of [1′-13C]inosine with F200G after (A) 40 min and (B) 24 h.

All 13C and 1H spectra were acquired with 5.3 mM [1′-13C]inosine and 6 μM PNP in the absence of phosphate at 25 °C in 10% D2O. [1] Ino, inosine; [5] N3-Ino, N3-inosine.