A) Photomicrograph montage of the dentate granule cell layer (gcl) in a transverse section of the dorsal hippocampus immuno-reacted against Kcna1 anti-serum, from a 4 month-old Kv1.1-infected knock-out mouse. The animal was sacrificed 3 days post-infection. Immunocytochemistry shows numerous Kv1.1-positive GCs (large arrows), as well as many weakly-stained GCs (expressing low levels of Kv1.1 IR) (arrowheads). A few GC axons (i.e., mossy fibers) were also stained (small arrows).
B) Higher magnification of a Kv1.1-infected GC. Kv1.1 channel protein is highly expressed in soma (arrow), dendrites (arrowheads) and axon (small arrow). Background staining is likely an artifact (also seen in ICC of Kv1.1−/− mouse tissue).
C) Photomicrograph of the CA3 pyramidal cell layer showing several Kcna1-immuno-labeled neurons (arrow).
D) Two-dimensional reconstruction of one of the CA3 pyramidal neurons from C (same animal as shown in A) showing Kv1.1 channel expression in soma (arrow), dendrites (arrowheads), and axon (small arrow).
Scale bars: A and C: 20μm; B and D: 10μm.