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. Author manuscript; available in PMC: 2009 Sep 27.
Published in final edited form as: Epilepsia. 2007 Jul 25;48(11):2023–2046. doi: 10.1111/j.1528-1167.2007.01189.x

Figure 8. Immunocytochemical evidence of Kv1.1 channel α subunits in infected neurons of Kv1.1 knock-out mouse.

Figure 8

A) Photomicrograph montage of the dentate granule cell layer (gcl) in a transverse section of the dorsal hippocampus immuno-reacted against Kcna1 anti-serum, from a 4 month-old Kv1.1-infected knock-out mouse. The animal was sacrificed 3 days post-infection. Immunocytochemistry shows numerous Kv1.1-positive GCs (large arrows), as well as many weakly-stained GCs (expressing low levels of Kv1.1 IR) (arrowheads). A few GC axons (i.e., mossy fibers) were also stained (small arrows).

B) Higher magnification of a Kv1.1-infected GC. Kv1.1 channel protein is highly expressed in soma (arrow), dendrites (arrowheads) and axon (small arrow). Background staining is likely an artifact (also seen in ICC of Kv1.1−/− mouse tissue).

C) Photomicrograph of the CA3 pyramidal cell layer showing several Kcna1-immuno-labeled neurons (arrow).

D) Two-dimensional reconstruction of one of the CA3 pyramidal neurons from C (same animal as shown in A) showing Kv1.1 channel expression in soma (arrow), dendrites (arrowheads), and axon (small arrow).

Scale bars: A and C: 20μm; B and D: 10μm.