FIG. 5.

(a) A plot of the theoretical magnetophoretic mobility of a 150 micron sphere as a function of the number of infused Dynabeads. These mobility numbers are quite high when compared to histogram plots of the negative (left histogram) and positive eluents (right histogram) from a immunomagnetic separation of human lymphocytes in a MiniMACS separation system (b). The cells were targeted with an anti-CD3 PE label and an anti-PE MACS reagent. Notice that 1.2 × 10⁴ MACS beads impart a single human lymphocyte with the same magnetophoretic mobility that a single 4.5 micron Dynabead gives a 150 micron sphere.