. Author manuscript; available in PMC: 2010 Sep 4.

Published in final edited form as: J Chromatogr A. 2009 Jul 10;1216(36):6365–6376. doi: 10.1016/j.chroma.2009.07.005

Table 1.

Model solutes and their characteristics. For the virus and the proteins, diameter was measured by DLS [1], for Na+ and Cl−, the given diameter is that of hydrated ions [2], and for UMP, the diameter was estimated from molecular structure.

Probe	Manufacturer	Cat. #	Lot #	MW	Hydrodynamic diameter (nm)	pI
NaCl (Na⁺, Cl⁻)	Fisher	S271	067655	(23, 35)	(0.72, 0.66)	n/a
uridine 5′-monophosphate (UMP)	Sigma	U6375	32H0433	324	1.3	n/a
ovalbumin	Sigma	A5503	076K7045	44,300	6	4.6
bovine serum albumin (BSA)	Sigma	A0281	075K7545	66,430	7	4.7
human adenovirus serotype 5 (Ad5)	Baylor College of Medicine, Vector Development Lab	Ad5-CMV-eGFP	102307, 030206	170,000,000	100	4.5