Figure 2.
Pseudorabies infection in the nucleus ambiguus.
A. Horizontal section, 50 μM thick, through the rat medulla showing immunostained PRV-GFP infected neurons in both the compact (NAc) and loose (NAl) portions of the nucleus ambiguus at four days after inoculation of the TA. Note the small number of NAl neurons (arrows) and infection of the compact portion without infection of the intervening semi-compact portion. Scale bar: 1mm.
B. High power micrograph of the NAc in a cresyl violet counterstained horizontal section showing (black) GFP-immunostained neurons typical of those consistently seen in this restricted portion of the compact NA four days after PRV-GFP inoculation of the TA. Note too the extensive gliosis and the ghost-like appearance of infected cells engulfed in glia (arrows). Scale bar: 100μM.
C. High power micrograph of a 50 μM thick section showing (brown) LacZ-immunostained neurons in the loose part of the NA following inoculation of PRV-LacZ into the posterior cricoarytenoid muscle (PCA). Scale bar: 50μM.
D. High power micrograph of the adjacent section to that shown in panel E but immunostained for both LacZ (brown) and GFP (black). PRV-GFP was injected at the same time into the TA as PRV-LacZ was injected into the PCA. Large motor neurons appear to be single-labeled but some smaller neurons (arrowheads in this and panel E) appear immunoreactive for both viruses and appear in both sections. Scale bar: 50μM.