Figure 6.

Ketamine effects on PV-interneurons do not require the presence of astrocytes. Primary neuronal cultures were grown on glass coverslips with “feet” as described (Kinney et al., 2006). After 21 d of development in vitro, the cultures were treated with ketamine (0.5 μm for 24 h) in the presence or absence of the astrocytic layer. For this, the coverslips containing neurons were separated from the astrocytic layer by transfer of the coverslip together with its media into an empty well. DHE was added for the last hour of treatment as described (Behrens et al., 2007). After treatment, neurons were fixed and processed for immunofluorescence for detection of either PV or GAD67 or for oxidized DHE. *^,#Statistical significant difference compared with control conditions at p < 0.001. n = 4–5 experiments per condition. Data are means ± SEM. Baseline intensities: PV = 210 ± 32; GAD67 = 195 ± 26.