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. Author manuscript; available in PMC: 2010 Aug 18.
Published in final edited form as: J Neuroimmunol. 2009 Jun 21;213(1-2):60–68. doi: 10.1016/j.jneuroim.2009.05.017

Fig. 3. MOG37-46 CD8+ T cells are low-avidity, pro-inflammatory, and polyclonal.

Fig. 3

Lymph node derived CD8+ T cells from animals primed with GP33-43, MOG37-46 or MOG44-54 were expanded in vitro with 10nM GP33-43, 10µM MOG37-46, or 10µM MOG44-54 for two weeks. A) IFNγ production in response to specific peptides was measured by ELISA. An average of three independent experiments is shown. Error bars represent standard deviation. B) After in vitro expansion with MOG37-46, lymph node derived CD8+ T cells were restimulated with 100µM of 37–46 for 5 hours in the presence of BFA and labeled with CD8+ and intracellular IFNγ, TNFα and IL-2. Analysis shown is gated on CD8+ cells. C) MOG37-46 in vitro expanded CD8+ T cells were labeled with anti-Vβ panel of antibodies; analysis is gated on CD8+ cells (C). Representatives of 3 independent experiments are shown (B and C).