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. Author manuscript; available in PMC: 2009 Sep 28.
Published in final edited form as: J Proteome Res. 2009 Jul;8(7):3642–3652. doi: 10.1021/pr800887u

Figure 2.

Figure 2

Gel electrophoresis and immunoblotting of Triton X-114-soluble membrane proteins from metacyclic trypomastigotes. Lanes 1 and 2, SDS-PAGE. Proteins were separated by SDS–PAGE on 10%-Laemmli gels and stained with Coomassie blue (lane 1) or silver (lane 2). Lanes 3 to 7, immunoblotting with monoclonal antibodies (mAb) against T. cruzi GPI-anchored surface proteins and flagellar calcium-binding protein (FCaBP) (lanes 3 to 6) and with a monospecific polyclonal antiserum against the catalytic domain of T. cruzi calpain-like cysteine proteinase (lane 7). The mAbs used were: 1G7 against GP90 surface glycoprotein (lane 3); 3F6 against GP82 surface glycoprotein (lane 4); 10D8 against the GP35/50 kDa mucin complex (lane 5); and 25 against the T. cruzi FCaBP (lane 6).