Table 1.
Statistics of phosphoproteome analysis of Trypanosoma cruzi epimastigote forms.
| Phosphoproteins |
||||||||
|---|---|---|---|---|---|---|---|---|
| MS approach | Non-phosphorylated proteins |
1P | 2P | 3P | 4P | >4P | Total phosphoproteins |
|
| MS/MS | 111 | 25 | 8 | 4 | 0 | 0 | 37 | |
| MS/MS/MS | 110 | 46 | 14 | 3 | 1 | 2 | 65 | |
| MSA | 80 | 60 | 22 | 11 | 5 | 1 | 99 | |
| Total | 156 | 74 | 30 | 18 | 5 | 4 | 131 | |
| T. cruzi | 139 | 72 | 24 | 16 | 3 | 4 | 119 | |
| Phosphopeptides | ||||||||
| Non-phosphorylated peptides | Phosphorylation sites | 1P | 2P | >2P | Total phosphopeptides | Reverse | FDR (%) | |
| MS/MS | 146 | 52 | 47 | 3 | 1 | 51 | 5 | 2.0 |
| MS/MS/MS | 124 | 65 | 75 | 5 | 4 | 84 | 9 | 4.1 |
| MSA | 96 | 162 | 146 | 9 | 5 | 160 | 9 | 3.4 |
| Total | 206 | 226 | 231 | 11 | 8 | 250 | 21 | 4.6 |
| T. cruzi | 196 | 221 | 227 | 5 | 5 | 237 | - | - |
| Distribution of T. cruzi phosphorylated sites |
||||||||
| Phosphoserine | Phosphothreonine | Phosphotyrosine | Ser or Thr | Ser or Tyr | ||||
| # of sites | 148 | 57 | 8 | 7 | 1 | |||
| % of Total | 65.5 | 25.2 | 3.5 | 3.1 | 0.4 | |||