Table 3.
Blast Analysis |
|||||||
---|---|---|---|---|---|---|---|
Hit # | Protein description | Accession # | P sites |
Sequence description | min. e-value | InterPro | Gene Ontology |
Signal transduction | |||||||
1 | regulatory subunit of protein kinase a-like protein | EAN92816.1 | 5 | regulatory subunit of protein kinase a-like protein | 1.0E-0.0 | Cyclic nucleotide-binding, RmlC-like jelly roll fold | cAMP-dependent protein kinase complex |
2 | phosphoprotein phosphatase | EAN90617.1 | 2 | serine threonine protein | 1.0E-0.0 | Serine/threonine-specific protein phosphatase and bis(5-nucleosyl)-tetraphosphatase | hydrolase activity |
3 | rac serine-threonine kinase | EAN95526.1 | 2 | serine threonine protein kinase | 1.0E-0.0 | Zinc finger, FYVE-type | ATP binding, protein amino acid phosphorylation, protein serine/threonine kinase activity, zinc ion binding |
4 | hypothetical protein | EAN95573.1 | 2 | WD repeat domain 17 | 1.0E-0.0 | WD40/YVTN repeat-like | none |
5 | protein kinase-A catalytic subunit | AAL17691.2 | 1 | protein kinase a catalytic subunit | 1.0E-0.0 | Serine/threonine protein kinase | protein serine/threonine kinase activity |
6 | hypothetical protein | EAN96688.1 | 1 | hypothetical protein | 1.0E-0.0 | WD40 repeat-like | none |
7 | hypothetical protein | EAN97518.1 | 1 | hypothetical protein | 1.0E-0.0 | WD40/YVTN repeat-like | none |
8 | hypothetical protein | EAN88571.1 | 1 | WD-repeat proteinexpressed | 1.0E-0.0 | WD40/YVTN repeat-like | nucleotide binding |
9 | calmodulin | EAN86242.1 | 1 | calmodulin | 1.00E-80 | Calcium-binding EF-hand | calcium ion binding |
10 | protein kinase (GSK3 beta) | EAN98751.1 | 1 | protein kinase | 1.0E-0.0 | Serine/threonine protein kinase-related | ATP binding, protein amino acid phosphorylation, protein serine/threonine kinase activity |
11 | hypothetical protein | EAN99395.1 | 1 | EF-hand family protein | 1.0E-0.0 | Calcium-binding EF-hand | calcium ion binding |
12 | hypothetical protein | EAN93781.1 | 2 | hypothetical protein | 1.0E-0.0 | Forkhead-associated | none |
13 | hypothetical protein | EAN91302.1 | 2 | chromosome 6 open reading frame 224 | 1.0E-0.0 | Shikimate kinase, Adenylate kinase, YHS | shikimate kinase activity, ATP binding |
14 | hypothetical protein | EAN97116.1 | 1 | calmodulin-related protein | 1.00E-180 | none | calcium ion binding |
15 | hypothetical protein | EAN83100.1 | 1 | EF-hand domain | 1.0E-0.0 | Region of unknown function DM10, Protein of unknown function DUF1126 | calcium ion binding, flagellum, |
16 | hypothetical protein | EAN83235.1 | 1 | hypothetical protein | 1.0E-0.0 | C2 calcium-dependent membrane targeting | none |
Cytoskeleton, flagellum and traffic proteins | |||||||
17 | paraflagellar rod component Par1b | AAC32103.1 | 3 | 69 kda paraflagellar rod protein | 1.0E-0.0 | Paraflagellar rod | calmodulin binding, microtubule-based flagellum |
18 | hypothetical protein | EAN99904.1 | 2 | hypothetical protein | 1.00E-134 | Major sperm protein, PapD-like | structural molecule activity |
19 | Beta tubulin 2.3 | AAL75957.1 | 1 | beta-tubulin | 1.0E-0.0 | Tubulin/FtsZ, GTPase | structural molecule activity |
20 | kinesin | EAN86063.1 | 1 | kinesin family member 14 | 1.0E-0.0 | Kinesin, motor region | microtubule motor activity, ATP binding, microtubule associated complex, microtubule-based movement |
21 | hypothetical protein | EAN84227.1 | 1 | dc2-related axonemal dynein intermediate chain | 1.0E-0.0 | none | none |
22 | dynein heavy chain | EAN97081.1 | 1 | dynein heavy chain | 1.0E-0.0 | ATPase associated with various cellular activities, AAA-5 | ATP binding, ATPase activity |
23 | clathrin coat assembly protein | EAN98227.1 | 1 | clathrin coat assembly protein | 1.0E-0.0 | ENTH/VHS, Epsin-like, N-terminal | phosphatidylinositol binding, clathrin coat, clathrin binding, clathrin cage assembly |
24 | hypothetical protein | EAN96083.1 | 1 | flagellar protein | 1.00E-154 | none | none |
25 | hypothetical protein | EAN88130.1 | 1 | Tctex1 domain | 1.00E-68 | Tctex-1 | flagellum, microtubule associated complex, microtubule-based process |
26 | hypothetical protein | EAN87422.1 | 1 | neurofilament heavy polypeptide (NF-H) | 1.0E-0.0 | none | structural molecule activity |
Transporters | |||||||
27 | ABC transporter | EAN96763.1 | 6 | ABC transporter | 1.0E-0.0 | ABC-2 type transporter | ATP binding, ATPase activity |
28 | ABC transporter | EAN92058.1 | 3 | ABC transporter | 1.0E-0.0 | ABC transporter-like | ATP binding, ATPase activity |
29 | ABC transporter | EAN89676.1 | 2 | ABC transporter | 1.0E-0.0 | ABC-2 type transporter | ATP binding, ATPase activity |
30 | metal-ion transporter | EAN85275.1 | 1 | zinc transporter-like protein | 1.0E-0.0 | Cation efflux protein | cation transport, cation transmembrane transporter activity, membrane |
31 | chloride channel protein | EAN94356.1 | 1 | chloride channel 7 | 1.0E-0.0 | Cystathionine beta-synthase, Chloride channel, voltage gated | voltage-gated chloride channel activity, chloride transport, membrane |
32 | calcium channel protein | EAN97848.1 | 1 | calcium channel protein | 1.0E-0.0 | Ion transport | ion channel activity, ion transport, membrane |
33 | GPR1/FUN34/yaaH family | EAN82352.1 | 1 | GPR1/FUN34/yaaH family (Acetate transporter) | 3.44E-135 | GPR1/FUN34/yaaH | membrane |
34 | Na+-ATPase | BAC98847.1 | 1 | calcium motive p-type ATPase | 1.0E-0.0 | ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter | ATP binding, membrane, cation transport, catalytic activity, metabolic process |
DNA, RNA and protein turnover | |||||||
35 | GTP-binding elongation factor Tu family | EAN90873.1 | 4 | GTP binding protein 1 | 1.0E-0.0 | Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal | GTPase activity, GTP binding |
36 | heterogeneous nuclear ribonucleoprotein H/F | EAN91678.1 | 4 | heterogeneous nuclear ribonucleoprotein h | 1.0E-0.0 | RNA recognition motif, RNP-1 | nucleic acid binding, nucleotide binding |
37 | mitochondrial DNA polymerase I protein D | EAN86491.1 | 3 | mitochondrial dna polymerase i protein | 1.00E-135 | DNA polymerase A | DNA binding, DNA-directed DNA polymerase activity, DNA replication |
38 | hypothetical protein | EAN95162.1 | 3 | hypothetical protein | 1.0E-0.0 | RNA recognition motif, RNP-1 | nucleic acid binding |
39 | eukaryotic translation initiation factor | EAN92887.1 | 2 | eukaryotic translation initiation factor | 1.0E-0.0 | Eukaryotic translation initiation factor 4E (eIF-4E) | RNA binding, translation initiation factor activity, cytoplasm, translational initiation |
40 | 25 kDa translation elongation factor 1-beta | EAN82455.1 | 1 | translation elongation factor 1-beta | 2.16E-79 | Translation elongation factor EF1B, beta and delta chains, guanine nucleotide | translation elongation factor activity |
41 | Tcc1a22.3 | AAL82703.1 | 1 | elongation factor 1-gamma | 1.0E-0.0 | Glutathione S-transferase, Thioredoxin fold | translation elongation, protein biosynthesis, |
42 | hypothetical protein | EAN84292.1 | 2 | hypothetical protein | 1.0E-0.0 | Signal recognition particle, SRP54 subunit, GTPase | GTP binding, SRP-dependent cotranslational protein targeting to membrane, 7S RNA binding, membrane |
43 | histone H2B | EAN83534.1 | 1 | histone H2B | 4.12E-58 | Histone H2B | nucleosome, DNA binding, nucleus, nucleosome assembly |
44 | hypothetical protein | EAN86654.1 | 1 | PAB1 binding protein | 1.0E-0.0 | Ataxin-2, N-terminal | mitochondrion, mRNA polyadenylation, cytoplasm, positive regulation of protein biosynthesis, polysome, nucleus |
45 | mitochondrial DNA polymerase I protein C | EAN88565.1 | 1 | mitochondrial dna polymerase i protein | 1.0E-0.0 | DNA-directed DNA polymerase, family A | DNA binding, DNA-directed DNA polymerase activity, DNA replication |
46 | ATP-dependent RNA helicase | EAN96171.1 | 1 | ATP-dependent rna helicase | 1.0E-0.0 | DNA/RNA helicase, DEAD/DEAH box type, N-terminal | nucleic acid binding, ATP binding, ATP-dependent helicase activity |
47 | tyrosyl or methionyl-tRNA synthetase | EAN96489.1 | 1 | tyrosyl-tRNA synthetase | 1.0–128 | Nucleic acid-binding, OB-fold | tRNA binding |
48 | glutamamyl carboxypeptidase | EAN85499.1 | 1 | acetylornithine deacetylase | 1.00E-152 | Peptidase M20, dimerisation | proteolysis, metallopeptidase activity, protein dimerization activity |
49 | calpain cysteine peptidase | EAN83138.1 | 1 | calpain-like cysteine peptidase | 1.0E-0.0 | Peptidase C2, calpain | calcium-dependent cysteine-type endopeptidase activity, intracellular, proteolysis |
50 | calpain-like cysteine peptidase | EAN88142.1 | 1 | calpain-like cysteine peptidase | 1.0E-0.0 | Peptidase C2, calpain | calcium-dependent cysteine-type endopeptidase activity, intracellular, proteolysis |
51 | calpain-like cysteine peptidase | EAN92790.1 | 1 | calpain-like cysteine peptidase | 1.00E-139 | Region of unknown function DUF1935 | proteolysis, calcium-dependent cysteine-type endopeptidase activity, intracellular |
52 | hypothetical protein | EAN99674.1 | 2 | hypothetical protein | 1.0E-0.0 | Zinc finger, CCCH-type | nucleic acid binding, zinc ion binding |
53 | hypothetical protein | EAN96152.1 | 3 | cysteine clan family c2 | 1.0E-0.0 | none | proteolysis, calcium-dependent cysteine-type endopeptidase activity, intracellular |
54 | nucleosome assembly protein | EAN95410.1 | 1 | nucleosome assembly protein | 1.00E-158 | Nucleosome assembly protein (NAP) | nucleus, nucleosome assembly |
Metabolism | |||||||
55 | Tyrosine Aminotransferase | 1BW0 | 3 | tyrosine aminotransferase | 1.0E-0.0 | 1-aminocyclopropane-1-carboxylate synthase | tyrosine transaminase activity |
56 | ribose-phosphate pyrophosphokinase | EAN92422.1 | 3 | ribose-phosphate pyrophosphokinase | 1.0E-0.0 | Phosphoribosyl pyrophosphokinase | nucleoside metabolic process, magnesium ion binding, ribose phosphate diphosphokinase activity, nucleotide biosynthetic process |
57 | pyruvate dehydrogenase E1 alpha subunit | AAD11551.1 | 2 | pyruvate dehydrogenase e1 component alpha | 1.0E-0.0 | Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, | metabolic process |
58 | pyruvate phosphate dikinase 2 | AAG12986.1 | 1 | pyruvate phosphate dikinase | 1.0E-0.0 | Pyruvate/Phosphoenolpyruvate kinase, catalytic core | kinase activity |
59 | phosphoribosylpyrophosphate synthetase | EAN97393.1 | 1 | phosphoribosylpyrophosphate synthetase | 1.0E-0.0 | Phosphoribosyl pyrophosphokinase | nucleoside metabolic process, magnesium ion binding, ribose phosphate diphosphokinase activity, nucleotide biosynthetic process |
60 | ribose-phosphate pyrophosphokinase | EAN95566.1 | 1 | ribose-phosphate pyrophosphokinase | 1.0E-0.0 | Phosphoribosyl pyrophosphokinase | nucleoside metabolic process, magnesium ion binding, ribose phosphate diphosphokinase activity, nucleotide biosynthetic process |
61 | hypothetical protein | EAN95143.1 | 1 | hypothetical protein | 1.0E-0.0 | Methyltransferase type 11 | metabolic process, methyltransferase activity |
62 | hypothetical protein | EAN95748.1 | 1 | had superfamily | 1.0E-0.0 | HAD-superfamily hydrolase, subfamily IIA, CECR5 | metabolic process, hydrolase activity |
Pathogenesis | |||||||
63 | trans-sialidase | EAN91292.1 | 3 | trans-sialidase | 1.0E-0.0 | Concanavalin A-like lectin/glucanase, subgroup | exo-alpha-sialidase activity, pathogenesis |
64 | trans-sialidase | EAN83746.1 | 2 | trans-sialidase | 1.0E-0.0 | Neuraminidase | sialidase activity, pathogenesis |
65 | trans-sialidase | EAN96241.1 | 2 | trans-sialidase | 1.0E-0.0 | Concanavalin A-like lectin/glucanase, subgroup | exo-alpha-sialidase activity, pathogenesis |
66 | trans-sialidase | EAN99282.1 | 1 | trans-sialidase | 1.0E-0.0 | Concanavalin A-like lectin/glucanase | exo-alpha-sialidase activity, pathogenesis |
67 | dispersed gene family protein 1 (DGF-1) | EAN86627.1 | 1 | dispersed gene family protein 1 (DGF-1) | 1.0E-0.0 | Parallel beta-helix repeat, Pectin lyase fold/virulence factor | none |
Binding | |||||||
68 | hypothetical protein | EAN97824.1 | 3 | c11orf60 protein | 1.0E-0.0 | protein binding | cytoplasm, nucleus |
69 | hypothetical protein | EAO00106.1 | 3 | hypothetical protein | 1.0E-0.0 | none | protein binding |
70 | hypothetical protein | EAN92411.1 | 3 | p25-alpha domain-containing | 1.00E-74 | P25-alpha | none |
71 | hypothetical protein | EAN89957.1 | 3 | hypothetical protein | 1.0E-0.0 | Leucine-rich repeat | protein binding |
72 | hypothetical protein | EAN85866.1 | 1 | hypothetical protein | 1.0E-0.0 | Tetratricopeptide-like helical, Sel1-like, CS domain | binding |
73 | hypothetical protein | EAN88376.1 | 1 | hypothetical protein | 1.0E-0.0 | C2 calcium-dependent membrane targeting | none |
74 | hypothetical protein | EAN96978.1 | 1 | hypothetical protein | 1.0E-0.0 | none | nucleic acid binding, zinc ion binding |
75 | hypothetical protein | EAN92548.1 | 1 | T-complex-associated testis expressed 1 | 1.0E-0.0 | Leucine-rich repeat | protein binding |
76 | I/6 autoantigen | EAN91318.1 | 1 | I/6 autoantigen | 1.00E-83 | calcium ion binding | structural constituent of cytoskeleton, cytoplasm, calcium ion binding, cytoskeleton, microtubule |
77 | I/6 autoantigen | EAN94637.1 | 1 | I/6 autoantigen | 1.00E-82 | calcium ion binding | structural constituent of cytoskeleton, cytoplasm, calcium ion binding, cytoskeleton, microtubule |
Other functions | |||||||
78 | retrotransposon hot spot (RHS) protein | EAN86249.1 | 2 | retrotransposon hot spotprotein | 1.0E-0.0 | SMAD/FHA domain | none |
79 | retrotransposon hot spot (RHS) protein | EAN99306.1 | 2 | retrotransposon hot spotprotein | 1.0E-0.0 | SMAD/FHA domain | none |
80 | retrotransposon hot spot (RHS) protein | EAN94997.1 | 1 | retrotransposon hot spotprotein | 1.0E-0.0 | SMAD/FHA domain | none |
81 | hypothetical protein | EAN88126.1 | 2 | isoform c | 1.0E-0.0 | CCR4-Not complex component, Not1 | muscle development, dendrite morphogenesis, sugar binding |
82 | hypothetical protein | EAN90373.1 | 2 | Mucoid inhibitor A | 1.0E-0.0 | Conserved hypothetical protein CHP02231 | none |
83 | hypothetical protein | EAN85046.1 | 1 | hypothetical multipass transmembrane protein | 1.0E-0.0 | none | none |
84 | peroxin 14 | EAN89658.1 | 1 | peroxin 14 | 1.0E-0.0 | Peroxisome membrane anchor protein Pex14p, N-terminal | peroxisome, membrane |
85 | hypothetical protein | EAN98546.1 | 1 | cg17097-isoform b | 1.0E-0.0 | none | none |
86 | hypothetical protein | EAN93909.1 | 1 | hypothetical multipass transmembrane protein | 1.0E-0.0 | none | none |
Proteins without known function or domain | |||||||
87 | hypothetical protein | EAN90722.1 | 5 | hypothetical protein | 1.0E-0.0 | none | none |
88 | hypothetical protein | EAN94368.1 | 5 | hypothetical protein | 1.0E-0.0 | none | none |
89 | hypothetical protein | EAN88585.1 | 4 | hypothetical protein | 1.0E-0.0 | none | none |
90 | hypothetical protein | EAN91358.1 | 3 | hypothetical protein | 1.00E-49 | none | none |
91 | hypothetical protein | EAN91901.1 | 3 | hypothetical protein | 1.0E-0.0 | none | none |
92 | hypothetical protein | EAN94097.1 | 3 | hypothetical protein | 1.00E-105 | none | none |
93 | hypothetical protein | EAN94312.1 | 3 | hypothetical protein | 1.0E-0.0 | none | none |
94 | hypothetical protein | EAN83060.1 | 2 | hypothetical protein | 2.83E-114 | none | none |
95 | hypothetical protein | EAN85364.1 | 2 | hypothetical protein | 2.46E-121 | none | none |
96 | hypothetical protein | EAN93581.1 | 2 | hypothetical protein | 1.00E-159 | none | none |
97 | hypothetical protein | EAN95093.1 | 2 | hypothetical protein | 1.0E-0.0 | none | none |
98 | hypothetical protein | EAN96967.1 | 2 | hypothetical protein | 1.00E-124 | none | none |
99 | hypothetical protein | EAN99066.1 | 2 | hypothetical protein | 1.0E-0.0 | none | none |
100 | hypothetical protein | EAN99070.1 | 2 | hypothetical protein | 1.00E-178 | none | none |
101 | hypothetical protein | EAN86750.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
102 | hypothetical protein | EAN87778.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
103 | hypothetical protein | EAN88004.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
104 | hypothetical protein | EAN90456.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
105 | hypothetical protein | EAN93004.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
106 | hypothetical protein | EAN93058.1 | 1 | hypothetical protein | 1.00E-70 | none | none |
107 | hypothetical protein | EAN82852.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
108 | hypothetical protein | EAN94518.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
109 | hypothetical protein | EAN94661.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
110 | hypothetical protein | EAN94862.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
111 | hypothetical protein | EAN98665.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
112 | hypothetical protein | EAN99075.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
113 | hypothetical protein | EAN99719.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
114 | hypothetical protein | EAN99770.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
115 | hypothetical protein | EAN99804.1 | 1 | hypothetical protein | 1.00E-176 | none | none |
116 | hypothetical protein | EAN96262.1 | 1 | hypothetical protein | 1.60E-93 | none | none |
117 | hypothetical protein | EAN80998.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |
118 | hypothetical protein | EAN86037.1 | 1 | hypothetical protein | 1.00E-67 | none | none |
119 | hypothetical protein | EAN91356.1 | 1 | hypothetical protein | 1.0E-0.0 | none | none |