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. 2009 Sep 8;7:35. doi: 10.1186/1477-5956-7-35

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Copyright © 2009 Gazzana and Borlak; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Proteome mapping of mouse liver. A pre-fractionation step prior to the IEF was carried out for the samples 1A-6A to separate proteins in two fractions: acid proteins with pH values < 7 (1A-a; 6A-a) and basic proteins having pH values > 7 (1A-b; 6A-b). All fractions were run in duplicate modus for the first and second dimensions. In summary, three experiments (E = 1, E = 3, E = 4) were repeated for the samples in the pH range 5-8. One experiment (E = 2) was carried out using the pH range 7-10. A total of 24 gels (grey small boxes) were used for cutting, digestion and MS identification of the spots/proteins. (N° = number; E = experiment; A-a = extract in LB2, acidic fraction; A-b = extract in LB3, basic fraction).