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. Author manuscript; available in PMC: 2010 Mar 6.
Published in final edited form as: J Mol Biol. 2009 Jan 8;386(4):938–950. doi: 10.1016/j.jmb.2008.12.080

Figure 2.

Figure 2

HDGF represses the expression of a one-hybrid reporter gene. The reporter gene G5-SV-LUC (Firefly luciferase 0.5 ug), pRL-CMV (Renilla luciferase 50 ng) and GBD-HDGF (0.5, 1, and 2 ug in left panel), or GBD (0.5, 1 and 2 ug in right panel) alone were cotransfected into G-7 cells. The dual-luciferase reporter assay system was used to measure the luciferase activities 24 hours after transfection. Percentage of firefly luciferase activity was calculated when luciferase activity with reporter gene alone was set at 100%. Firefly luciferase activity was normalized with constitutive renilla luciferase activity to correct transfection efficiency. Bars indicate mean ± SEM from 3 separate transfection assays with duplicate plates, *P < 0.05 by one-way ANOVA control versus GBD-HDGF.