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. Author manuscript; available in PMC: 2009 Sep 28.
Published in final edited form as: FASEB J. 2007 Sep 12;22(2):455–465. doi: 10.1096/fj.07-8276com

Figure 1.

Figure 1

EGF up-regulates PAI-1 gene expression in glioblastoma cells. A172 and U373 cells were treated with EGF or IL-1 for 7 h (A); RNA was isolated and subjected to Northern blot analysis using PAI-1 cDNA as a probe. A172 cells were treated with EGF for indicated time periods (B) or pretreated with 5 µg/ml actinomycin D for 30 min, then treated with EGF for 7 or 18 h (C), and processed as in A. D) A172 cells were left untreated or treated with EGF for 18 h, and then 5 µg/ml actinomycin D was added for indicated time periods and processed as in A. The lower panels show 28S RNA stained with ethidium bromide on the membrane as a loading control.

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