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. Author manuscript; available in PMC: 2010 Sep 30.
Published in final edited form as: Virology. 2009 Jul 30;392(2):246–259. doi: 10.1016/j.virol.2009.07.001

Figure 6. PIC activates microglial TLR3.

Figure 6

Microglia were transfected with TLR3-specific siRNA or control siRNA at 10 or 30 nM for 3D and then stimulated with PIC for 6h, as described in the Materials and Methods. Transcripts for TLR3, IFNβ, IDO and viperin were measured by real time PCR with PBDA as a control. Silencing microglial TLR3 with specific siRNA (more effective at 10 nM) reduced microglial expression of all four genes. The suppression of viperin was less effective. Data are normalized to the transcript levels present in PIC-treated, control-siRNA transfected microglia. These results show that PIC activates microglia primarily through TLR3.