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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Dev Biol. 2009 Jul 24;334(1):243–252. doi: 10.1016/j.ydbio.2009.07.028

Figure 2. Le-Cre mediated deletion of Cited2 results in abnormal corneal morphology and increased corneal epithelial proliferation.

Figure 2

Histological examination was performed by the H&E staining of eye sections collected from 6.5-week old mice. Compared to the normal morphology of the Cited2flox/flox control cornea (a), thinner corneal epithelium and fewer corneal epithelial cells were observed in Cited2flox/flox;Le-Cre+ cornea (b). PAS staining was also performed in conjunction with H&E staining and revealed intact basement membrane in Cited2flox/flox control cornea (arrow in c), but not in Cited2flox/flox;Le-Cre+ cornea (d). Ki-67 immunostaining (red color) and DAPI counterstain (blue color) further showed increased Ki-67 positive cells (pink color merged from Ki-67 and DAPI staining) in Cited2flox/flox;Le-Cre+ corneal epithelium (arrows in f) compared to the few Ki-67 positive corneal basal cells detected in Cited2flox/flox control (arrowheads in e) (CEP: corneal epithelium; CS: corneal stroma; CE: corneal endothelium).