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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2009 Aug 11;8(8):2204–2210. doi: 10.1158/1535-7163.MCT-09-0160

Figure 3. NVP-BEZ235 causes cell cycle arrest and induces autophagy.

Figure 3

(A) Cell cycle analysis was performed on cells treated or untreated with 50 nM NVP-BEZ235 for 48 h. Cell cycle distribution was labeled on the histogram. NVP-BEZ235 treatment resulted in G1 cell cycle arrest. (B) Following NVP-BEZ235 treatment, glioma cells were either stained with acridine orange for FACS analysis, to assess autophagy, or harvested for immunoblotting analysis, to assess LC3-II expression. Top panel shows the results of FACS analysis of acridine-positive cells. It appears that glioma cells are prone to NVP-BEZ235-induced autophagy. Bottom panel shows LC3-II expression as an indication of autophagy in the (−) untreated and (+) NVP-BEZ235-treated cells.