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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Cancer Res. 2009 Sep 1;69(17):6915–6923. doi: 10.1158/0008-5472.CAN-09-0664

Figure 1. Downregulation of SphK1 expression or its inhibition by SK1-I reduces growth of glioma cells.

Figure 1

(A,B) U373 and LN229 cells transfected with control siRNA or siRNA targeted to SphK1. (A) Cells were cultured for the indicated time in the absence or presence of 5% serum and cell growth was determined with WST-1. (B) Equal amounts of lysates were separated by SDS-PAGE and immunoblotted with anti-SphK1 antibody. Blots were stripped and re-probed with anti-clathrin heavy chain (CHC) antibody to confirm equal loading and transfer. * indicates non-specific band. SphK1 activity was measured in naïve U373 and LN229 cells. (C) U373 and LN229 cells were cultured for the indicated time in the absence or presence of SK1-I (3 and 10 μM) without or with 5% serum. Cell growth was determined by WST1 assay.