Figure 6. SK1-I enhances survival of mice with LN229 cells implanted intracranially.

(A–D) LN229-H2B-GFP cells were implanted into the brain of nu/nu mice 1 mm lateral to the Bregma and at a depth of 3.5 mm. Twenty days later, tumor bearing mice were randomized into two groups and injected i.p. with saline or SK1-I (10 mg/kg) every other day until day 49. (A) Representative MRI images at day 48 are shown: T2-weighted images of vehicle and SK1-I treated animals. Note the dramatic effect of SK1-I on tumor growth compared to the massive tumor in untreated animal. (B) Contrast-enhanced T1-weighted image of SK1-I treated animal. (C) Kaplan Meier survival curves for animals implanted with LN229 cells treated with vehicle or SK1-I, as described above. (D) Intracranial tumors of vehicle and SK1-I treated animals were visualized by confocal microscopy for GFP expression (green) after counterstaining with Hoechst 33342 (blue). Representative sections at the site of injection are shown. Scale bars, 100 microns.