Figure 1.

The rod bipolar cell transduction current is blocked by antagonists of TRPV1. A, Response to 100 μm LY341495 (delivered via fast-flow apparatus) before (left) and after (center) a 5 min application of 10 μm ruthenium red. Right, Response of another cell to a 1 s puff of LY341495 delivered through a puffer pipette alone (top) or during simultaneous application of ruthenium red from a second puffer pipette (middle). Ruthenium red was applied alone for 10 s before obtaining the middle trace. The inhibition of ruthenium red was readily reversed using this approach (bottom). Calibration: 10 pA, 2 s. B, Response to LY341495 before and after a 5 min application of 100 μm 2-APB. C, D, Responses to 1 s puffs of LY341495 (100 μm) before and after 5 min bath application of 20 μm capsazepine (C) and 20 μm SB366791 (D). Responses to LY341495 typically showed partial recovery after removal of antagonists, as shown in the right panel of D. Traces in C and D are from different cells. E, Summary of results. The number of cells for each experiment is indicated above each bar.