FIGURE 4.
Spatial Profile of the Evanescent Illumination in the x–y Plane. (a) Evanescently excited Alexa Fluor 488 dye was non-specifically adsorbed to the surface of fused silica. The circle indicates the observed area within the pinhole. (b) Data were obtained by slicing images with x = 0 or y = 0 and plotting the corresponding pixel intensities as a function of distance. A representative slice with y = 0 and its best fit to Eq. 24 is also shown.