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. Author manuscript; available in PMC: 2010 Apr 9.
Published in final edited form as: J Phys Chem B. 2009 Apr 9;113(14):4837–4845. doi: 10.1021/jp810642d

FIGURE 4.

FIGURE 4

Spatial Profile of the Evanescent Illumination in the x–y Plane. (a) Evanescently excited Alexa Fluor 488 dye was non-specifically adsorbed to the surface of fused silica. The circle indicates the observed area within the pinhole. (b) Data were obtained by slicing images with x = 0 or y = 0 and plotting the corresponding pixel intensities as a function of distance. A representative slice with y = 0 and its best fit to Eq. 24 is also shown.