Table 1. qRT-PCR failed to replicate array data on right/left miRNAs asymmetry in the brain.
Array data | qRT-PCR data | |||||
miRNA | Region | R/L FC | P | Adj. P | R/L ratio | P |
let-7a | hip | 0.67 | 0.007 | 0.52 | 1.08 | 0.95 |
hyp | 1.70 | 0.0003 | 0.04 | 0.71 | 0.73 | |
miR-132 | hip | 0.73 | 0.02 | 0.78 | 0.99 | 0.68 |
hyp | 1.39 | 0.01 | 0.48 | 0.91 | 0.62 | |
miR -320 | hip | 0.66 | 0.005 | 0.52 | 1.16 | 0.52 |
hyp | 1.42 | 0.02 | 0.66 | 0.95 | 0.42 | |
miR -448 | cb | 0.46 | 0.03 | 1.00 | nd | nd |
hip | 2.18 | 0.04 | 0.96 | nd | nd | |
miR -497 | cb | 1.61 | 0.02 | 1.00 | nd | nd |
hip | 1.60 | 0.02 | 0.96 | nd | nd |
cb: cerebellum; hip: hippocampus; hyp: hypothalamus; R/L FC: Fold change between the right and left hemisphere of the given region; P: nominal P-value; Adj. P: Adjusted P-value corrected for multiple testing by BH; R/L ratio: Ratio of the relative miRNA expression levels in the right and left hemispheres, nd: not done.