TABLE 1.
Bacterial strains and plasmidsa
| Strain(s) or plasmid | Genotype or relevant characteristic(s) | Reference |
|---|---|---|
| Strainsb | ||
| FRD1 | CF isolate, mucoid | 15 |
| PAO1 | Wound isolate, nonmucoid | 9 |
| FRD1aceA (LS1539) | FRD1aceA101::aacCI | 11 |
| PAO1aceA (LS1545) | PAO1aceA101::aacCI | 11 |
| FRD1aceA+ (JH168) | FRD1aceA complemented for aceA | 11 |
| PAO1aceA+ (JH166) | PAO1aceA complemented for aceA | 11 |
| FRD1dadA (LS1760) | FRD1dadA101::aacCI | This study |
| PAO1dadA (LS1761) | PAO1dadA101::aacCI | This study |
| FRD1dadA+ (LS1794) | FRD1dadA complemented in cis | This study |
| PAO1dadA+ (LS1796) | PAO1dadA complemented in cis | This study |
| Plasmids | ||
| pLS214 | moriT present in pUC19 | 28 |
| pLS1755 | dadA101::aacCI in pBK+ | This study |
| pLS1793 | dadA complementing plasmid | This study |
| pLS1155 | Regulatable promoter/repressor system | 24 |
a
Abbreviations used for genetic markers are described by Holloway et al. (33).
b
Alternate strain names are shown in parentheses.