TABLE 2.
Primers and probes used in this study
| Primer or probe no. | Designation | Sequence (5′-3′)a |
|---|---|---|
| Primers | ||
| 1 | guaAF | GATTTTGGATCCCAATATAGCC |
| 2 | guaAR | CCCATTCTATGGTTGATGGAGGCTTAGA |
| 3 | guaA16159-SalIF | GTCGACGGATGGAATTGTAGGCCG |
| 4 | guaA15926-SalIRC | GTCGACGTAAACACTGGATTGTTGCGC |
| 5 | guaAcompF-SalI | GCGTCGACGGTTTATAGCTAGATCTTTTGATTTGGC |
| 6 | guaAcompRC-SalI | GCGTCGACTCAGCAGAATTTGCAGATGTATTCCC |
| 7 | flgBPo-XhoI | TAATACTCGAGCTTCAAGGAAGATTT |
| 8 | 3′ aacC1-NheI | GCTAGCCGATCTCGGCTTGAACG |
| 9 | flaB TaqMan FWD | TCTTTTCTCTGGTGAGGGAGCT |
| 10 | flaB TaqMan REV | TCCTTCCTGTTGAACACCCTCT |
| 11 | guaA TaqMan FWD | GAAGAATTAGAGAAATTGGCGTTTATAC |
| 12 | guaA TaqMan REV | GGTAGGAGCTTCTTTTGAATAAACAG |
| 13 | guaB TaqMan FWD | AGGAATAGGTCCGGGTAGTATATGC |
| 14 | guaB TaqMan REV | TCATAGACATCGCAGATTGCTGTT |
| 15 | ospC1-F | ACGGATTCTAATGCGGTTTTACCT |
| 16 | ospC1-R | CAATAGCTTTAGCAGCAATTTCATCT |
| 17 | guaA 3′ KpnI | GGGGTAACCTTATTATTCCCATTCTATGGTTGATGGAGGC |
| Probes | ||
| 1 | flaB TaqMan probe | 6-FAM-AAACTGCTCAGGCTGCACCGGTTC-TAMRA |
| 2 | guaA TaqMan probe | 6-FAM-AGCAGGACTTCCACTTAGTATTATTCCTGAGATATTCAT-TAMRA |
| 3 | guaB TaqMan probe | 6-FAM-AACTCCAACTCCCGCAACGATTCTTGTT-TAMRA |
| 4 | ospC1 probe | 6-FAM-CTGTGAAAGAGGTTGAAGCGTTGCTGTCAT-TAMRA |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.