FIG. 4.

The PBPRA3229 gene and the E. coli diaA gene can both complement the growth and morphological alterations of the P. profundum SSR FL23 mutant. (A and B) Cultures of the strains shown were grown in marine broth supplemented with streptomycin, diluted, and spotted onto low-gelling-temperature marine agar overlay plates supplemented with l-arabinose (0.01%, wt/vol) but not streptomycin. The data sets shown are representative of at least three independent experiments where similar growth patterns were observed. Due to the fragile nature of the overlay plates after incubation at high pressure, the plates could only be photographed in the plastic pouches. The imperfections in the photographs are due to deformations of the plastic pouches. (C to J) Cultures were diluted 1:500 in either marine broth alone (C to F) or with streptomycin and 0.01% (wt/vol) l-arabinose (G to N) and grown for ∼40 h under the conditions shown. The images shown are representative fields of view from at least two independent experiments. Scale bars = 10 μm.