FIG. 5.

The P. profundum SS9 PBPRA1039 gene partially complements the DNA replication defect of an E. coli mutant lacking SeqA and suppresses the cold sensitivity phenotype of an E. coli dnaA(Cs) mutant. (A to D) Exponential-phase cells of the strains shown were grown in the presence of streptomycin, thymine, and 0.2% (wt/vol) l-arabinose and fixed, and the chromosome number was determined with SYTOX Green in combination with flow cytometry. The profiles shown are representative of two independent experiments. (E and F) The strains shown were grown overnight in LB medium at 42°C supplemented with streptomycin and thymine but without l-arabinose. Cultures were then diluted; spotted onto LB agar supplemented with thymine, streptomycin, and 0.2% (wt/vol) l-arabinose; and then incubated under the conditions shown for 40 h. The data set shown is representative of at least two independent experiments where similar growth patterns were observed.